Feeder cell free culture method

Glycosaminoglycans (GAGs) enable sufficient stem cell culture process providing an artificial niche for improved cell growth

19040751 ml

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The primary invention of Glycostem, using specific extracellular matrix proteins such as Glycoasminoglycans, enables a highly efficient expansion culture system for stem cells.

The stroma free cell culture system using specific modified Glycosaminoglycans (GAGs) and growth factor/cytokines. The culture system is based on the formation of an artificial niche in a liquid cell culture system using GAGs in addition to a basic serum free medium, cytokine mixtures and human serum. GAGs as components of the extra cellular matrix are able to interact with growth factors, cell surface receptors and cations. The figure shows a model of the theoretical interaction between GAGs and cells by forming a structure mediated by the presentation of growth factors to the cellular receptors and by a direct binding to integrin or selectin cell adhesion receptors.